Discovering a Dihydrofluorescein Analogue as a Promising Fluorescence Substrate to HRP
نویسندگان
چکیده
Horseradish peroxidase (HRP) combined with its fluorescence substrates is attracting increasing attention for biochemical analysis. Amplex red the most widely used substrate to HRP; however, it suffers from some drawbacks, such as nonspecific responsiveness toward carboxylesterases. Discovering a new small molecular improved sensitivity and selectivity HRP thus desired. Herein, three dihydrofluorescein derivatives (DCFHs) are presented serve through turn-on methods. The promising one, 2,7-dichloro-9-(2-(hydroxymethyl)phenyl)-9H-xanthene-3,6-diol (DCFH-1), exhibited excellent in detection of HRP. Moreover, DCFH-1 does not respond carboxylesterase, holding advantages over red. In further study, reagent commercial ELISA kits was replaced establish ELISA, which works very well quantification inflammatory cytokine biomarkers vitro models.
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ژورنال
عنوان ژورنال: Chemosensors
سال: 2023
ISSN: ['2227-9040']
DOI: https://doi.org/10.3390/chemosensors11020152